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Axonal/Dendritic Growth and Circuit Formation

開催日 2014/9/12
時間 14:00 - 15:00
会場 Poster / Exhibition(Event Hall B)

Caspase 6-dependent synapse remodeling in the developing chick ciliary ganglion

  • P2-096
  • 加藤 秀理 / Hidetaka Katow:1 江川 遼 / Egawa Ryo:1 細島 頌子 / Hososhima Shoko:1 石塚 徹 / Ishizuka Toru:1,2 八尾 寛 / Yawo Hiromu:1,2,3 
  • 1:東北大学大学院生命科学研究科 / Tohoku University Graduate School of Life Sciences, Sendai, Japan 2:CREST, JST / CREST, JST 3:東北大学大学院医学系研究科 脳神経科学コアセンター / Center for Neuroscience, Tohoku University Graduate School of Medicine, Sendai, Japan 

Caspase 6-dependent synapse remodeling in the developing chick ciliary ganglion

Hidetaka Katow1, Ryo Egawa1, Shoko Hososhima1, Toru Ishizuka1,2, Hiromu Yawo1,2,3.
1:Graduate School of Life Sciences, Tohoku University, Sendai 2:CREST, JST 3:Center for Neuroscience, Tohoku University Graduate School of Medicine

The excess terminal branches of a presynaptic axon are generally removed during developmental stages of CNS as well as PNS (small-scale axon pruning, fine tuning). As a result, the neurons are connected with their appropriate peripheral targets. However, the molecular mechanisms underlying the small-scale axon pruning remained unrevealed. It has been hypothesized that caspase-3 and -6, that are involved in apoptosis-related cascades, are also the mediators of the axon pruning. The ciliary ganglion (CG) of chick embryo has been a model system of the small-scale axon pruning, and a presynaptic neuron of Edinger-Westphal nucleus terminates on the postsynaptic neuron in a one-to-one manner with a giant calyx. In this study, the caspase hypothesis is tested using CG of chick embryo. The plasmids were made for either the overexpression experiments or the dominant-negative experiments of each caspase-3 and -6. They were incorporated by midbrain neurons through in ovo electroporation at E2. On E12-14, the terminal branching was morphologically investigated under 3D confocal microscopy for tdTomato, of which expression was under regulation of sparse-expression system. Each axon with overexpressed caspase-3 formed a single calyx terminal in a one-to-one manner. On the other hand, some axons with dominant-negative caspase-6 branched in the ganglion to form multiple calyx terminals even at E14, a stage where the axonal branches are mostly eliminated. It is suggested that that caspase-6 is involved in the presynaptic axon pruning in the developing CG.

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