• Top page
  • Timetable
  • Per session
  • Per presentation
  • How to
  • Meeting Planner



パーキンソン病とその類縁疾患 2
Parkinson's Disease and Related Disorders 2

開催日 2014/9/12
時間 18:10 - 19:10
会場 Room I(311+312)
Chairperson(s) 野元 正弘 / Masahiro Nomoto (愛媛大学大学院医学系研究科 薬物療法・神経内科学 / Department of Neurology and Clinical Pharmacologu, Ehime University, Graduate School of Medecine, Japan)
村田 美穂 / Miho Murata (国立精神・神経医療センター 病院・神経内科 / Department of Neurology, National Center Hospital, National Center of Neurology and Psychiatry, Japan)

Immunohistochemistry of mouse and human brain using specific antibodies against oxidized DJ-1

  • O2-I-6-2
  • 斎藤 芳郎 / Yoshiro Saito:1 宮坂 知宏 / Tomohiro Miyasaka:2 初田 裕幸 / Hiroyuki Hatsuta:3 仁木 加寿子 / Kazuko Takahashi-Niki:4 有賀 寛芳 / Hiroyoshi Ariga:4 村山 繁雄 / Shigeo Murayama:3 井原 康夫 / Yasuo Ihara:2 野口 範子 / Noriko Noguchi:1 
  • 1:同志社大・生命医・システム生命科学 / Sys Life Sci Lab, Fac of Life Med Sci, Doshisha Univ, Kyotanabe, Japan 2:同志社大・生命医・神経病理学 / Neuropathol, Fac of Life Med Sci, Doshisha Univ, Kyotanabe, Japan 3:都健康長寿医療セ・神経病理 / Dep of Neuropathol, Tokyo Metro Inst of Geront, Tokyo, Japan 4:北大院・薬・分子生物学 / Dept of Mol Biol Lab, Grad Sch of Parm Sci, Hokkaido Univ, Sapporo, Japan 

DJ-1, the product of a causative gene of a familial form of Parkinson's disease (PD), undergoes preferential oxidation of cysteine residue at position 106 (Cys-106) under oxidative stress. Using specific antibodies against Cys-106-oxidized DJ-1, we examined oxidized DJ-1 immunoreactivity (oxDJ-1 IR) in wild type and DJ-1 knockout mouse brain sections as well as human brain sections from cases classified into different Lewy body (LB) stages of PD and PD with dementia. In mouse brain, the oxDJ-1 IR was particularly enriched in the olfactory bulb, cerebellum, cortex, and hippocampus. The oxDJ-1 IR was evident in the substantia nigra of the midbrain of mouse and human. In neuromelanin-containing neurons and nerve fibers of the substantia nigra of human brain section, oxDJ-1 IR was prominently observed, and the LBs also showed oxDJ-1 IR. OxDJ-1 was detected in the astrocytes of the striatum. In addition, neurons and glias in the red nucleus of the midbrain and the inferior olivary nucleus of the medulla oblongata, which is related to the regulation of movement, showed clear oxDJ-1 IR. The biochemical properties of oxDJ-1 in frozen brain samples were further investigated using oxDJ-1 specific antibodies. Consistent with immunostaining, oxDJ-1 band intensity in high oxDJ-1 IR group were significantly higher than that in the tissues from low oxDJ-1 IR group. Our observations suggest the relevance of DJ-1 oxidation to the homeostasis in multiple sites of brain, including neuromelanin-containing neurons of the substantia nigra.

Copyright © Neuroscience2014. All Right Reserved.