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Glia and Glia-Neuron Interaction

開催日 2014/9/13
時間 14:00 - 15:00
会場 Poster / Exhibition(Event Hall B)

Transformation of resident microglia into phagocytic cells in transected and cycloheximide-administered rat facial nucleus

  • P3-026
  • 島川 大輝 / Daiki Shimakawa:1 長嶺 保子 / Yasuko Nagamine:1 越本 茉亜紗 / Maasa Koshimoto:1 中川 美智子 / Michiko Nakagawa:1 高坂 新一 / Shinichi Kohsaka:2 中嶋 一行 / Kazuyuki Nakajima:1 
  • 1:創価大学 工学研究科 生命情報工学専攻 / Department of Bioinformatics, Faculty of Engineering, Soka University 2:国立精神・神経医療研究センター / National Institute of Neuroscience 

We analyzed the properties of microglia to transform into phagocytes in rat facial nucleus by administering a protein synthesis inhibitor cycloheximide at the transected facial nerve. Immunoblotting revealed that a phagocytic marker CD68 protein is induced in the transected and cycloheximide-applied facial nucleus at 5 days post-insult, while not significantly induced in either normal facial nucleus or simply transected facial nucleus. In the transected and cycloheximide-administered facial nucleus, the levels of choline acetyltransferase (ChAT) were significantly reduced, suggesting the functionally damaged state of motoneurons. The time course experiment indicated that the levels of CD68 protein peak at 5-7 days after transection/cyclohexmide-administration. Avidin-biotin peroxidase complex staining method clarified that the CD68-expressing cells appear at transected and cyclohexamide-administered facial nucleus, and they locate around motoneuron cell bodies. Fluorescent double staining method revealed that CD68-expressing cells are all positive for ionized Ca2+ binding adapter molecule 1 (a microglial marker). Collectively, these results demonstrated that the resident microglia have an ability to transform into phagocytic cells in response to injured/cycloheximide-administered motoneurons in the facial nucleus.

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