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Reproduction, Feeding and Metabolic Regulation

開催日 2014/9/12
時間 10:00 - 11:00
会場 Room J(313+314)
Chairperson(s) 功刀 浩 / Hiroshi Kunugi (独立行政法人国立精神・神経医療研究センター 神経研究所 / Department of Mental Disorder Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Japan)
上田 陽一 / Yoichi Ueta (産業医科大学医学部 第2生理学 / Department of Physiology, School of Medicine, University of Occupational and Environmental Health, Japan)

A novel sexually dimorphic area exhibiting female-dominant morphology in the hypothalamus of mice

  • O2-J-2-2
  • Kyi Tha Thu Chaw:1 Hiroto Ito:1 Kota Okoshi:1 Ken-ichi Matsuda:2 Mitsuhiro Kawata:2 Shinji Tsukahara:1 
  • 1:Laboratory of Regulatory Biology, Division of Life Science, Graduate School of Science and Engineering, Saitama University, Japan 2:Department of Anatomy and Neurobiology, Kyoto Prefectural University of Medicine Graduate School of Medical Science, Japan 

Testicular androgen during perinatal period is a key molecule to organize sexually dimorphic nuclei (SDNs) in rodent brain, and it is aromatized in the brain and binds with estrogen receptor α (ERα) to exert an influence on SDNs formation. In this study, we examined the brain of transgenic mice, which express green fluorescent protein (GFP) under the control of ERα promoter, and wild-type mice; consequently, we discovered a sexually dimorphic area in the hypothalamus of mice. First, we examined calbindin-D28K (Calb)-immunostained brain sections of the transgenic mice and observed that females had many more GFP-expressing neurons than males in an area sandwiched between two Calb-immunoreactive SDNs, the principal nucleus of the bed nucleus of the stria terminalis (BNSTp) and the Calb-SDN. Next, we studied the sandwiched area in wild-type mice and found that the sandwiched area contained larger number of neurons in females than in males, but no sex difference in the number of glia cells was found. We also examined ERα-immunostained brain sections of wild-type mice and found that ERα-immunoreactive cell number was greater in females than in males. To evaluate the effects of postnatal androgen on formation of male-typical sandwiched area, we examined adult males bearing neonatal castration and adult females that were injected with either estradiol or testosterone in the postnatal period. As a result, neuron number of the sandwiched area was significantly increased in neonatal castrated males, while postnatal treatment with estradiol and testosterone significantly decreased neuron number of females. These results suggest that an area sandwiched between the BNSTp and Calb-SDN of the mouse brain is a novel female-dominant sexually dimorphic area and that testicular androgen in the postnatal period has a defeminizing effect on the sandwiched area.

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