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Cerebrovascular Disease and Ischemia

開催日 2014/9/13
時間 11:00 - 12:00
会場 Poster / Exhibition(Event Hall B)

The Association between neurogenesis and functional recovery after cerebral ischemia

  • P3-337
  • 城本 高志 / Takashi Shiromoto:1 岡部 直彦 / Naohiko Okabe:1 陸 豊 / Fung Lu:1 中村-丸山 恵美 / Emi Nakamura-Maruyama:1 氷見 直之 / Naoyuki Himi:1 成田 和彦 / Kazuhiko Narita:1 木村 和美 / Kazumi Kimura:2 宮本 修 / Osamu Miyamoto:1 
  • 1:川崎医科大学 生理学2 / Dept Physiol, Kawasaki medical school, Okayama, Japan 2:日本医科大学 神経内科 / Dept Neurology, Nippon Medical School, Tokyo Japan 

Previous study has shown that rehabilitative therapy (RT) increases neurogenesis in the subventricular zone (SVZ). But the functional effect of neurogenesis in SVZ is not fully understood. To investigate the association between neurogenesis and functional recovery,it is essential to know precise time course of neurogenesis after cerebral ischemia (CI). In the present study, we examined two preliminary examinations. In the first experiment, we examined the effect of RT on functional recovery and motor map reorganization using skilled forelimb reaching test and intracortical microstimulation. In the second experiment, the time course study of neurogenesis after CI was conducted. Further, effects of RT on neurogenesis were also examined.
Adult male rats were subjected to focal CI divided into RT group and a non- RT group. CI was induced in the caudal forelimb area using the photothrombosis model. RT was carried out by continuing the skilled forelimb training for 4weeks after operation.
Neurogenesis was analyzed using immunohistochemistry. Bromodeoxyuridine(BrdU) was injected on the day before perfusion to detect proliferating neural stem cells. In time course study, rats were perfused at 2, 4, 7, 14, and 28 days after cerebral ischemia. Nestin and BrdU-labeled cells were counted in the SVZ areas.
In the first experiment, RT significantly improved functional recovery and increased rostral forelimb area.
In the second experiment, the neurogenesis increased from 2 days after CI and the peak value was attained at 7days after CI. Then, the number of Nestin and Brdu double labelled cells were gradually decreased and returned to normal value at 28 days after CI. In the RT group, a significant increase of neurogenesis was observed compared to the non- RT group.
As previously reported, RT enhanced functional recovery and cerebral cortex plasticity in our experiment. And RT increased neurogenesis after CI. In time course study, enhanced neurogenesis was pronounced in the first week, suggesting the inhibition of neurogenesis in first week after CI would be suitable to investigate the association between neurogenesis and functional recovery.

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