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開催日 2014/9/13
時間 11:00 - 12:00
会場 Poster / Exhibition(Event Hall B)

Non-invasive manipulation of neuronal activities by optogenetic activation of ChR2-expressing astrocytes

  • P3-377
  • 吉田 慶多朗 / Keitaro Yoshida:1 徐 明 / Ming Xu:1 三村 將 / Masaru Mimura:1 高田 則雄 / Norio F Takata:1 田中 謙二 / Kenji F Tanaka:1 
  • 1:慶応大学 / Dept. Neuropsychiatry, School of Medicine, Keio University 

The functions of brain can be studied in two main ways. Firstly, the changes in the brain's state can be measured using techniques such as EEG or functional MRI. Modern imaging techniques such as CT, fMRI and NIRS provide non-invasive measurement of neuronal activities. Secondly, the activity of the brain can be manipulated through the use of brain stimulation. However, the requirements of both time-controlled activation and non-invasive stimulation make the application difficult. Optogentics is known for the high spatial and temporal resolution that it provides in altering the cell type specific activity. But an optic fiber insertion to brain parenchyma causes an injury, which is easy to evoke glial cell activation and change their function. We thus challenged to establish an experimental model that satisfies above points. The usage of light-sensitive channelrhodopsin variant, C128S, permitted successful photoactivation of cortical astrocytes by an illumination over the skull, resulting in consistent neuronal activation. Thus, we established an experimental model that permitted successful manipulation of neuronal activities by non-invasive optogenetic approach. We further demonstrated that a weak illumination resulted in the focal activation but a strong illumination resulted in the propagation of neuronal activities throughout the hemisphere.

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