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Sensorimotor Learning/Plasticity

開催日 2014/9/12
時間 11:00 - 12:00
会場 Poster / Exhibition(Event Hall B)

Transcranial imaging of cortical activities after sound-shape association in mice

  • P2-125
  • 山岸 達矢 / Tatsuya Yamagishi:1,2 塚野 浩明 / Hiroaki Tsukano:1 鎌谷 大樹 / Daiki Kamatani:1 菱田 竜一 / Ryuichi Hishida:1 髙橋 姿 / Sugata Takahashi:2 澁木 克栄 / Katsuei Shibuki:1 
  • 1:新潟大・脳研・システム脳生理 / Dept Neurophysiol, Brain Res Inst, Niigata Univ, Niigata, Japan 2:新潟大・医・耳鼻咽喉科 / Dept Otolaryngol, Sch Med, Niigata Univ, Niigata, Japan 

We can recall a particular shape from the sound stimulus intimately associated with the shape. To investigate this sound-shape association memory in mice, we developed an M-shaped maze equipped with a screen and a speaker. First, a combination of sound (A) and shape (A), or sound (B) and shape (B) was presented to water-deprived mice. After that, shape (A) and shape (B) were presented at the two inlets of the M-maze branches. If the mice choose the branch with the same shape presented before, they could obtain a small amount of water. This trial was repeated 20 times per each day. In this visually-guided task, mice learned to select the correct shapes after about 20 sessions of 20 trials. To test sound-shape association memory, we further tested whether the mice could choose the shapes based on the associated sound cues only. In this association memory-guided task, we confirmed that wild-type mice could select the correct shapes based on the sound cues only. Clustered protocadherins (cPcdhs) are neuron-specific cell adhesion molecules with multiple clusters. Wild type mice have 12 clusters (α1-α12) of cPcdh-α, while, cPcdh-α1,12 mice have only α1 and α12 clusters of cPcdh-α. Although cPcdh-α1,12 mice could learn the visually-guided task, their performance in the association memory-guided task was significantly worse compared with that in wild-type mice. To investigate neural mechanisms underlying the sound shape association memory, we investigated cortical responses to the sound stimuli using flavoprotein fluorescence imaging in the trained wild-type mice. The cortical responses to the sound stimuli were observed in the auditory cortex and the higher visual cortices located dorsally to the auditory cortex. The activation of the higher visual cortices after the sound stimuli may play an important role in sound-shape association memory.

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