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Olfaction, Taste, Chemical Senses

開催日 2014/9/13
時間 14:00 - 15:00
会場 Poster / Exhibition(Event Hall B)

The neural representation of salt taste in the rat chorda tympani nerve studied by using stereotrode method

  • P3-146
  • 菅野 菜月 / Natsuki Sugano:1 峯積 博之 / Hiroyuki MINEZUMI:1 小林 哲宏 / Tetsuhiro Kobayashi:1 宮川 博義 / Hiroyoshi Miyakawa:1 井上 雅司 / Masashi Inoue:1,2 
  • 1:東京薬科大学 生命科学部 脳神経機能学研究室 / Laboratory of Cellular Neurobiology, Tokyo University of Pharmacy and Life Science 2:モネル化学感覚センター / Monell Chemical Senses Center, Philadelphia PA, USA 

The salty taste is elicited by ionized salts. The cations of the salts, especially sodium cations, are mainly responsible for the salty taste, but the anions also contribute to a lesser extent. Although the epithelial sodium channel (ENaC) expressed in the taste receptor cells has been reported as a key molecule that elicit salty taste, there are ENaC independent pathways which have not been well characterized. We have previously reported a novel method using a stereotrode that enables to record multiple single units simultaneously in the chorda tympani (CT) nerve. Here we have tried to explore how the salt taste information is represented in the taste nerve by using the stereotrode method. The anesthetized rat CT nerve was desheathed and teased into several fine strands. Each strand was placed on a glass platform and two electrodes made from glass and/or nichrome wire were inserted into the strand. Off-line spike sorting was carried out using EToS (Efficient Technology of Spike sorting) software based on principal component measurement of the units. We used two measures of unit isolation quality, L ratio and Isolation Distance to qualify cluster separation and set selection criteria as L < 0.1 and Distance > 3.0. We have examined responsiveness of each isolated unit to lingual stimuli consisting of various ionized solutions with or without amiloride, a blocker of ENaC. We have observed both amiloride-sensitive and insensitive units and they have shown various types of responsiveness to both the cations and the anions that have been tested. We will discuss the information representation of the salty taste in the CT nerve.

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