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Trophic Factors

開催日 2014/9/12
時間 18:10 - 19:10
会場 Room J(313+314)
Chairperson(s) 小島 正己 / Masami Kojima (独立行政法人産業技術総合研究所 健康工学研究部門 バイオインターフェース研究グループ / Biointerface Research Group, Health Research Institute (HRI), National Institute of Advanced Industrial Science and Technology (AIST), )
篠田 陽 / Yo Shinoda (東京理科大学理工学部 応用生物科学科 / Deparment of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Japan)

Retrograde BDNF signaling from Purkinje cell regulates synapse elimination in the developing cerebellum

  • O2-J-6-4
  • 秋 明貞 / Myeong Jeong Choo:1 宮崎 太輔 / Taisuke Miyasaki:2 山崎 真弥 / Maya Yamazaki:3 谷村 あさみ / Asami Tanimura:1 上阪 直史 / Naofumi Uesaka:1 渡辺 雅彦 / Masahiko Watanabe:2 崎村 健司 / Kenji Sakimura:3 狩野 方伸 / Masanobu Kano:1 
  • 1:東京大院医神経生理 / Dept Neurophysiol, Univ of Tokyo, Tokyo, Japan 2:北海道大院医解剖発生 / Dept Anat, Grad Sch Med, Hokkaido Univ, Sapporo, Japan 3:新潟大・脳研・細胞神経生物 / Dept Cell Neurobiol, Brain Res Inst, Niigata Univ, Niigata, Japan 

Precise formation of connections between neurons is prerequisite for highly sophisticated brain function. Elimination of redundant synapses formed at earlier developmental stage is known as "synapse elimination" and is considered to be an essential process in neural circuit formation. The climbing fiber (CF) to Purkinje cell (PC) synapse in the cerebellum provides an ideal model for the study of developing synapse elimination. In the neonatal mouse cerebellum, each PC is innervated by multiple CFs. During postnatal development, early-formed surplus CFs are gradually eliminated and most PCs eventually become innervated by single CFs in mature mice. However, molecular mechanisms of CF synapse elimination still remain largely unknown. In this study, we examined whether brain-derived neurotrophic factor (BDNF) in postsynaptic PCs is involved in CF synapse elimination using conditional knockout mice in which BDNF is deleted in Purkinje cells (BDNF-PC-KO mice). In mutant mice, about 40% of PCs were innervated by multiple (two or three) CFs while only 20% of PCs were innervated by two CFs in control mice from postnatal day 21 (P21). These results suggest that BDNF signaling from postsynaptic Purkinje cell regulates developmental synapse elimination in the cerebellum.

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