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アルツハイマー病、他の認知症、老化 3
Alzheimer's Disease, Other Dementia, Aging 3

開催日 2014/9/12
時間 15:00 - 16:00
会場 Room I(311+312)
Chairperson(s) 玉岡 晃 / Akira Tamaoka (筑波大学医学医療系神経内科学 / Department of Neurology, University of Tsukuba, Japan)
田口 明子 / Akiko Taguchi (宮崎大学医学部神経呼吸内分泌代謝学分野 / Department of Neurology, Respirology, Endocrinology and Metabolism, Miyazaki University School of Medicine, Japan)

Contribution of preoptic area thermo transient receptor potential vanilloid type IV (TRPV4) channel in thermoregulation in rats

  • O2-I-3-1
  • Rajesh Yadav:1 Hruda Nanda Mallick:1 Ashok kumar Jaryal:1 Velayudhan Kumar 
  • 1:All India Institute of Medical Sciences, New Delhi, India 

Method: The study was conducted in 4 male Wistar rats. Under thiopentone sodium anesthesia (40 mg/kg BW) a bilateral guide cannula (24G) with indwelling styli was implanted with their tips aimed at 2 mm above the preoptic area (POA) as per De Groot's atlas. A radio transmitter TA10TAF-40 (Data Science International, USA) for the telemetric recording of body temperature (Tb) was implanted in the abdomen. A K- type thermocouple wire was inserted near the hypothalamus to measure the brain temperature (Tbr). Tbr was recorded at 15 second interval through a fluke digital thermometer. Tb was recorded telemetrically at 15 second interval. The temperature was measured from 10.00 to 16.00 h and injection was given at 12.00 h. Temperature data was averaged at 15 minute epochs. TRPV4 agonist,(GSK1016790A,(0.2μg/0.2μl) injection was given bilaterally at the POA at a rate of 0.1 micro liter /minute using an injector cannula. The site of injection was confirmed histologically. The statistical comparison was made between control and post injection record at every 15 minutes using paired t-test.

Results: The body temperature recorded in five rats range between 37.04 ± 0.2ºC to 37.74 ± 0.26ºC and brain temperature 36.413± 0.31ºC to 36.87 ± 0.21ºC. The injection of TRPV4 agonist,(GSK1016790A, (0.2μg/0.2μl) into preoptic area produced a prompt fall in body and brain temperature. Tbr significantly decreased from 36.83±0.12ºC to 36.491±0.08ºC, 12:00 to 12:15h, (p<0.005), 36.825 ± 0.19ºC to 36.465±0.13ºC, 12:15 to 12:30 h, (p<0.005), 36.866 ± 0.35ºC to 36.31 ± 0.23ºC, 12:30 to 12:45 h,(p<0.05), 36.729±0.19ºC to 36.203±0.31ºC 12:45 to 13:00 h, (p<0.005), 36.654 ±0.19ºC to 36.128± 0.39ºC, 13:00 to 13:15 h, (p<0.05), 36.707 ± 0.18ºC to 36.126 ± 0.51 ºC, 13.15 to 13:30 h, (p < 0.05) and Tb significantly decreased from 37.587 ± 0.44ºC to 37.212 ± 0.3ºC, 12:00 to 12:15 h, (P<0.05), 37.442 ± 0.4ºC to 36.943 ± 0.3ºC, 12:15 to 12:30 h, (P<0.05).

Conclusion: The TRPV4 channel agonist injection in the preoptic area (POA) brings about fall in body and brain temperature by stimulating warm sensitive neurons.

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