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ポリグルタミン病、ALS、脊髄小脳変性症、その他の神経変性疾患 2
Polyglutamine Diseases, ALS, SCD, Other Neurodegenerative Disorder 2

開催日 2014/9/11
時間 10:00 - 11:00
会場 Room I(311+312)
Chairperson(s) 柳 茂 / Shigeru Yanagi (東京薬科大学 生命科学部 分子性化学研究室 / Laboratory of Molecular Biochemistry, School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Japan)
三澤 日出巳 / Hidemi Misawa (慶應義塾大学薬学部 薬理学 / Department of Pharmacology, Faculty of Pharmacy, Keio University, Japan)

Spatiotemporal alteration of phosphatidylcholines in a mouse model of amyotrophic lateral sclerosis

  • O1-I-2-2
  • 有馬 秀幸 / Hideyuki Arima:1 大村 威夫 / Takao Omura:1 早坂 孝宏 / Takahiro Hayasaka:2 正木 紀隆 / Noritaka Masaki:2 花田 充 / Mitsuru Hanada:1 坂野 友啓 / Tomohiro Banno:1 徐 冬びん / Dongmin Xu:1 長谷川 智彦 / Tomohiko Hasegawa:1 小林 和克 / Kazuyoshi Kobayashi:3,4 竹内 英之 / Hideyuki Takeuchi:5 門松 健治 / Kenji Kadomatsu:4 松山 幸弘 / Yukihiro Matsuyama:1 瀬藤 光利 / Mitsutoshi Setou:2 
  • 1:浜松医科大学 整形外科 / Department of Orthopaedic surgery, Hamamatsu University School of Medicine, Japan 2:浜松医科大学 解剖学講座細胞生物学分野 / Dept Cell Biology and Anatomy, Hamamatsu University School of Medicine, Shizuoka, Japan 3:名古屋大学 整形外科 / Dept Orthopedic Surgery, Nagoya University Graduate School of Medicine, Aichi, Japan 4:名古屋大学大学院医学系研究科生物化学講座 / Dept Biochemistry, Nagoya University Graduate School of Medicine, Aichi, Japan 5:名古屋大学 環境医学研究所 / Research Institute of Environmental Medicine Nagoya University, Aichi, Japan 

Recently, imaging mass spectrometry (IMS) has emerged as a powerful technique to visualize spatial distributions of various molecules in situ. In the present study, we aimed to analyze changes of lipid composition characteristic with time in the spinal cord of an ALS mouse model using IMS.
Animal experiment protocols were approved by the Ethical Committee of the Hamamatsu University School of Medicine. Female SOD1G93A mice were used as ALS models and their littermates were used as controls. Lumbar spinal cords were collected from 5 before onset), 10(early stage after onset), and 22 weeks (terminal stage after onset) old mice, respectively. Ten micrometer-thick frozen sections of L5 lumbar spinal cords were assessed by IMS, visualizing molecular species of phosphatidylcholine (PC).
The IMS results showed significant decrease of docosahexaenoic acid such as (DHA)-containing PCs, PC (diacyl-16:0/22:6), PC (diacyl-18:0/22:6), and PC (diacyl-18:1/22:6) in the L5 anterior horns of 22 week-old SOD1 G93A mice. PC (diacyl-16:0/16:0) was observed specifically in the L5 dorsal horn grey matter irrespective to age and genotype. There were no significant difference in the spatiotemporal distribution of arachidonic acid-containing PCs between groups.
Our data revealed a significant decrease in DHA-containing PCs at the terminal stage of ALS mice, which may reflect neuronal loss in the anterior horns of the spinal cords in ALS mice. The preservation of PC (diacyl-16:0/16:0), which was specifically distributed in the dorsal horn, suggested that the afferent nerves in the lumbar spinal cords were not affected in ALS mice. These findings indicate that ALS spinal cord may show significant alterations in PC metabolism only at the terminal stage of disease.

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