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Stem Cells, Neuronal and Glial Production/Differentiation

開催日 2014/9/11
時間 16:00 - 17:00
会場 Poster / Exhibition(Event Hall B)

Chemical library screening to identify a small compound that promotes motor neurons differentiation from iPSCs/ESCs

  • P1-070
  • 後藤 和也 / Kazuya Goto:1,2 今村 恵子 / Keiko Imamura:2,3 三谷 幸之介 / Kohnosuke Mitani:4 饗庭 一博 / Kazuhiro Aiba:5 中辻 憲夫 / Norio Nakatsuji:5 髙橋 良輔 / Ryosuke Takahashi:1 井上 治久 / Haruhisa Inoue:2,3 
  • 1:京都大院医臨床神経 / Department of Neurology, Kyoto University, Kyoto, Japan 2:京都大iPS細胞研増殖分化機構研究部門 / Laboratory of Stem Cell Medicine, Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan 3:JST, CREST / Japan Science and Technology Agency (JST), Core Research for Evolutional Science and Technology (CREST), Japan 4:埼玉医大ゲノム医学セ遺伝子治療 / Division of Gene Therapy, Research Center for Genomic Medicine, Saitama Medical University, Saitama, Japan 5:京都大iCeMS / Institute for Integrated Cell-Material Sciences (iCeMS), Kyoto, Japan 

Stem cell technology, using induced pluripotent stem cells (iPSCs) or embryonic stem cells (ESCs), provides us with human spinal motor neurons (MNs), which had previously been inaccessible, to investigate MN diseases including amyotrophic lateral sclerosis. However, limitations including low efficiencies remain in the currently available MN differentiation protocols that hamper the use of human iPSCs/ESCs.
We designed a chemical library screening with the aim of identifying a molecule that promotes differentiation of MNs from iPSCs/ESCs. We validated the screening assay, and set up screening flows using HB9-GFP knock-in human iPSCs/ESCs based on a conventional MN differentiation protocol. This study demonstrates that a phenotypic assay using reporter stem cell lines would be used for a large-scale compound screening for MN differentiation.

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