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Neurotransmitters, Gliotransmitters, and Modulators

開催日 2014/9/12
時間 11:00 - 12:00
会場 Poster / Exhibition(Event Hall B)

The elevation of intracellular Zn2+ concentration by dopamine in cultured cortical neurons

  • P2-019
  • Hui-Hsing Hung:1 Chien-Yuan Pan:1 
  • 1:National Taiwan University 

Dopamine is one of the most important neurotransmitters in brain. Our previous studies have shown that dopamine treatment elevates the intracellular Zn2+ concentration ([Zn2+]i) in PC12 cells and elicits autophagosome formation. We sought to further characterize the effects of dopamine-induced [Zn2+]i elevation on neurotransmission in cultured cortical neurons. Neurons expressing the autophagosome marker GFP-LC3 were treated with dopamine (250 µM) to induce the formation of autophagosomes; TPEN, a Zn2+ chelator, was used to block the dopamine-induced [Zn2+]i elevation to verify the correlation between [Zn2+]i elevation and autophagosome formation. Our results showed a significant increase in the number of autophagosomes in neurons treated with dopamine; TPEN pretreatment inhibited this effect. In addition, dopamine treatment suppressed the synaptic transmission among neurons; blocking dopamine-induced [Zn2+]i elevation with TPEN reversed the inhibition. These data suggest that dopamine regulates neuron transmission by modulating the [Zn2+]i level; however, the mechanism how dopamine induces [Zn2+]i elevation is not clear. We will use different inhibitors to characterize how dopamine modulate the [Zn2+]i level to verify the importance of Zn2+ in neurotransmission.

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