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Visual System

開催日 2014/9/13
時間 11:00 - 12:00
会場 Poster / Exhibition(Event Hall B)

Electrophysiological and behavioral approaches to understanding the neural basis of color vision in Drosophila melanogaster

  • P3-113
  • 関 洋一 / Yoichi Seki:1 中村 菜々美 / Nanami Nakamura:1 米倉 太郎 / Taro Yonekura:1 山田 陵平 / Ryohei Yamada:2 新田 遥 / Haruka Nitta:1 宮川 博義 / Hiroyoshi Miyakawa:1 森本 高子 / Takako Morimoto:1 
  • 1:東薬大生命 / Laboratory of Cellular Neurobiology, School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Tokyo, Japan 2:東工大院総合理工 / Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, Kanagawa, Japan 

Many animals including insects can discriminate color based on the activities of several photoreceptor classes that have a distinct spectral sensitivity. However, neural mechanisms underlying color vision are not yet fully understood. Drosophila melanogaster offers a good model system for deciphering the neural basis of color vision due to the numerical simplicity of the circuits and the availability of rich genetic tools. In this study, we aimed to establish electrophysiological recordings from CNS neurons of the fly brain involved in color vision. To examine spectral sensitivities from the neurons in the color processing pathway, we first set up the light stimulus system that can give monochromatic lights ranging from 300-700 nm wavelengths. We evaluated the system by using electroretinograms and examined several basic conditions critical for recordings such as stimulus intensity and interval. We are currently working on establishing whole-cell patch-clamp recordings from second-order and third-order neurons in the color processing pathway. In addition to the electrophysiological approach, we aimed to evaluate the color discrimination ability of Drosophila using a behavioral experiment. We used an appetitive conditioning, where flies were given R/G/B light from the iPad LCD display as a conditioned stimulus (CS) and sucrose as a reward (unconditioned stimulus; US). We discuss how these approaches can be combined to understand the neural basis of color vision.

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