Thalamus was traditionally viewed as a component of neural circuits in generalized seizures, and recent studies have suggested that thalamus also plays a role in temporal lobe epilepsy (TLE), but how thalamus affects the evolution of temporal lobe epileptic seizures is unclear. In this study, local filed potentials (LFPs) and single-unit activities were obtained from CA1 of hippocampus and parafascicular nucleus of thalamus during development of epileptic seizures induced by pilocarpine in mice. Combined computational methods from symbolic dynamics and information theory, redundancy and directionality index, were used to analyze the electrophysiological characters of neuronal activities and information flow between thalamus and hippocampus. We found that LFPs became more regular during the seizure in both hippocampus and thalamus, and in some cases LFPs showed a transit disorder at seizure onset. The variation tendency of the peak values of cross-correlation function between neurons matched the variation tendency of redundancy of LFP. The information tended to flow from thalamus to hippocampus during seizure initiation period, no matter what the information flow direction was before the seizure. In some cases the information flow was symmetrically bidirectional, but none was found in which the information would flow from hippocampus to thalamus during the seizure initiation period. In addition, inactivation of thalamus by tetrodotoxin (TTX) resulted in suppression of seizures. These findings suggest that thalamus may play an important role in the initiation of epileptic seizures.