Intraneuronal transport is fundamental mechanism for survival, morphogenesis, and function of neurons. This transport is affected in several neurodegenerative disorders. We have developed an automated monitoring system for axonal transport in primary cultured chick dorsal root ganglion (DRG) neurons. This system utilizes chloromethylbenzamide dialkycarbocyanine (CM-DiI) to visualize membranous organelles, which are transported along the axons. We here applied this system to assess the intraneuronal transport in iCell®Neurons, which are derived from human iPS cells. Histograms of instantaneous velocity of anterograde and retrograde intraneuronal transport of iCell®Neurons showed a bimodal distribution pattern. We further assessed the effects of anti-neoplastic drugs on intraneuronal transport of iCell®Neurons. Vincristine and paclitaxel suppressed anterograde and retrograde intraneuronal transport significantly. But Cisplatin and oxaliplatin did not produce the effects on intraneuronal transport of iCell®Neurons, although these drugs suppressed axonal transport of chick DRG neurons. Our system may be useful for evaluation of intraneuronal transport in human neurons. In addition, this system will be applied for studying neurodegenerative disorders.