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Staining, Tracing, and Imaging Techniques

開催日 2014/9/12
時間 11:00 - 12:00
会場 Poster / Exhibition(Event Hall B)

Computational analysis of the effects of anti-neoplastic agents on intraneuronal transport of human iPSC derived neurons

  • P2-391
  • 中村 治子 / Haruko Nakamura:1,4 山下 直也 / naoya yamashita:1 金丸 悠理 / yuri kanamaru:2 関野 祐子 / yuko sekino:3 後藤 敏行 / toshiyuki gotoh:2 田中 章景 / fumiaki tanaka:4 五嶋 良郎 / yoshio goshima:1 
  • 1:横浜市立大学 大学院医学研究科 分子薬理神経生物学 / Department of Molecular Pharmacology and Neurobiology, Yokohama City University Graduate School of Medicine, Yokohama, Japan 2:横浜国立大学大学院環境情報学府情報メディア環境学専攻 / Graduate School of Environment and Information Sciences, Yokohama National University, Yokohama, Japan 3:国立医薬品食品衛生研究所 薬理部 / Division of Pharmacology, National Institute of Health Sciences, Tokyo, Japan 4:横浜市立大学 大学院医学研究科 神経内科学・脳卒中医学 / Department of Neurology and Stroke Medicine, Yokohama City University Graduate School of Medicine, Yokohama, Japan 

Intraneuronal transport is fundamental mechanism for survival, morphogenesis, and function of neurons. This transport is affected in several neurodegenerative disorders. We have developed an automated monitoring system for axonal transport in primary cultured chick dorsal root ganglion (DRG) neurons. This system utilizes chloromethylbenzamide dialkycarbocyanine (CM-DiI) to visualize membranous organelles, which are transported along the axons. We here applied this system to assess the intraneuronal transport in iCell®Neurons, which are derived from human iPS cells. Histograms of instantaneous velocity of anterograde and retrograde intraneuronal transport of iCell®Neurons showed a bimodal distribution pattern. We further assessed the effects of anti-neoplastic drugs on intraneuronal transport of iCell®Neurons. Vincristine and paclitaxel suppressed anterograde and retrograde intraneuronal transport significantly. But Cisplatin and oxaliplatin did not produce the effects on intraneuronal transport of iCell®Neurons, although these drugs suppressed axonal transport of chick DRG neurons. Our system may be useful for evaluation of intraneuronal transport in human neurons. In addition, this system will be applied for studying neurodegenerative disorders.

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