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Learning and Long-term Memory

開催日 2014/9/13
時間 11:00 - 12:00
会場 Poster / Exhibition(Event Hall B)

Roles of histone modifications in formation and regulation of fear memory

  • P3-217
  • 天野 翔次郎 / Shojiro Amano:1 喜田 聡 / Satoshi KIDA:1,2 
  • 1:東京農業大学大学院 / Dept. of Bioscience, Tokyo Univ. of Agriculture 2:科学技術振興機構CREST / CREST, JST 

Memory consolidation is a process to stabilize short-term memory (STM), generating long-term memory (LTM). A critical biochemical feature of memory consolidation is a requirement of gene expression. Chromatin remodeling by histone acetylation, methylation and phosphorylation plays important roles in regulation of transcription. Importantly, recent studies have suggested that chromatin remodeling through histone modifications is required for memory formation. To understand roles of histone modifications in memory regulation at the anatomical level, we tried to identify brain regions in which histone modifications are induced during consolidation of contextual fear memory using immunohistochemistry. In contextual fear conditioning test, mice were trained with (Shock+) or without (Shock-) an electrical footshock. We observed significant increase in the number of positive cells of S10 phosphorylation and K14 acetylation of histone H3 (H3S10pK14ac), a marker of transcriptional activation, in the hippocampus of Shock+ group 90 min after the conditioning compared to control groups. In contrast, we did not observe changes in H3S10pK14ac-positive cells in medial prefrontal cortex, anterior cingulate cortex and amygdala regions following the conditioning. Furthermore, we also did not observe changes in the number of positive cells of acetylation of K9 in histone H3 (H3K9ac) in the hippocampus. Our results suggest that H3S10pK14ac in the hippocampus plays important roles in formation of contextual fear memory.

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