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Axonal/Dendritic Growth and Circuit Formation

開催日 2014/9/11
時間 11:00 - 12:00
会場 Poster / Exhibition(Event Hall B)

Expression of lysosomal-associated membrane protein 5 (Lamp5) gene in cerebellum

  • P1-103
  • 古戎 道典 / Michinori Koebis:1 齊藤 由弦 / Yuzuru Saito:1 篠田 陽 / Yo Shinoda:1 古市 貞一 / Teiichi Furuichi:1,2 
  • 1:東京理科大院理工応用生物科学 / Dept Appl Biol Sci, Tokyo Univ of Sci, Chiba, Japan 2:理研BSI発生遺伝子制御 / Lab for Developmental Gene Regulation, RIKEN BSI, Saitama, Japan 

In the mammalian central nervous system (CNS) more than a half of the genes in the genome are expressed in a spatiotemporally regulated manner. Although they are thought to be involved in brain developments and functions, most of them remain to be studied for the subcellular localizations and molecular functions of their encoded proteins in the CNS. When they are expressed in a specific stage of the brain development or in a specific cell type or brain region, the genes should be involved in the neural development and function, such as differentiation of neurons, neural network formation, and synaptic plasticity. Recently, we have developed Cerebellar Development Transcriptome Database (CDT-DB) (Sato et al., Neural Netw, 2008), and identified several brain-specific genes whose encoded proteins have not yet been characterized for their functional significance in the CNS including the gene encoding lysosomal-associated membrane protein 5 (LAMP5, also known as BAD-LAMP). We found that Lamp5 is highly expressed in embryonic mouse cerebellum and is down-regulated in later developmental stages while its expression at the whole brain level increases along with the development. The nematode orthologous gene of Lamp5, unc-46, encodes a type III transmembrane protein with several glycosylation sites. UNC-46 has been shown to locate in the synaptic terminal of GABAergic neurons and regulate the release of GABA, an inhibitory neurotransmitter that plays an important role in brain development. A previous study also showed that LAMP5 protein was expressed in the II/III and V layers of mouse postnatal cerebral cortex. However, its expression over other brain regions during developmental stages is still unknown. In the present study, we have performed immunohistochemistry of murine brain sections at various developmental stages with a polyclonal antibody against LAMP5 and found its expression in cerebellum and a certain cell population, which is presumably located near the locus coeruleus. We are now generating a Lamp5 knockout mouse to investigate the physiological function of LAMP5 in the CNS.

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