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Parkinson's Disease and Related Disorders

開催日 2014/9/11
時間 11:00 - 12:00
会場 Poster / Exhibition(Event Hall B)

Repression of neuronal cell death by secreted DJ-1 from astrocytes

  • P1-297
  • 仁木 加寿子 / Kazuko Takahashi-Niki:1 綿引 佑実 / Yumi Watahiki:1 北浦 廣剛 / Hirotake Kitaura:1 仁木 剛史 / Takeshi Niki:2 有賀 寛芳 / Hiroyoshi Ariga:1 
  • 1:北海道大学院薬 / Grad Sch Pharm Sci, Hokkaido Univ 2:北海道大院農 / Grad Sch Agr, Hokkaido Univ 

DJ-1 was first identified by our group as a novel oncogene. Deletion and point mutations of DJ-1 have been shown to be responsible for onset of familial Parkinson's disease(PD). DJ-1 plays many functions. DJ-1 is located in the cytoplasm, nucleus and mitochondria in cells, and secreted DJ-1 has been observed in various cultured cells and tissues, including cancer cells and tissues, astrocytes and serum of patients with Parkinson's disease. But, the mechanism of DJ-1 secretion is still unkown.
Previously, we reported that DJ-1 was found to be secreted into culture media of various cells. Furthermore, the oxidative status of more than 90% of the DJ-1 secreted from cells was SOH and SO2H forms of cysteine at 106(C106).
In this study, the effect of secreted DJ-1 from astrocytes on viability of neurons and production of reactive oxygen species(ROS) was examined in the non-contact co-cultured system of astrocytes an neurons. The results showed that astrocytes from DJ-1(+/+) mouse but not from DJ-1(-/-) mouse restored neuroprotective activity that has reduced under an oxidative condition and that secreted wild-type DJ-1 but not C106S from DJ-1-transfeted astrocytes into culture media protects H2O2-induced neuronal cell death and decreased production of ROS in neurons.

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