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Spinal cord Motor Neurous and Muscle

開催日 2014/9/11
時間 11:00 - 12:00
会場 Poster / Exhibition(Event Hall B)

Hyperglycemia causes the reduction of numbers and sizes of abdominal motoneurons in STZ rats

  • P1-125
  • 大城 直美 / Naomi Ohshiro:1 村松 憲 / Ken Muramatsu:2 佐々木 誠一 / Sei-ichi Sasaki:3 丹羽 正利 / Masatoshi Niwa:1 
  • 1:杏林大学 / Kyorin University 2:健康科学大学 理学療法学科 / Department of Physical Therapy,Health Science University 3:茨城県立医療大学 医科学センター / Center for Medical Sciences,Ibaraki Prefectural University of Health Sciences 

Hyperglycemia causes the reduction of numbers and sizes of abdominal motoneurons in STZ rats

Oshiro Naomi 1, Ken Muramatsu 2, Sei-Ichi Sasaki 3, Masatoshi Niwa 1

1 Department of Occupational Therapy, Kyorin University
2 Department of Physical Therapy, Health Science University
3 Center for Medical Sciences, Ibaraki Prefectural University of Health Sciences

Diabetic peripheral polyneuropathy (DPN) is a major complication in diabetic patients, but it had been believed that motoneurons are relatively protected from DPN. We recently observed that there is a significant decrease in the absolute number and size of medial gastrocnemius (MG) motoneurons, especially in the smaller motoneurons, in diabetic rats and reported that it is highly possible that DPN predominantly targets smaller motoneurons of the distal muscle. However, also the functional disorder of abdominal (Abd) muscles, postural and breathing disorder, is pointed out in diabetic patients. In this study we examined alterations in the number and size of motor nucleus of Abd muscles using experimental type I diabetic rats.
Type I diabetes was induced in 13-week-old male Wistar rats with streptozotocin (STZ, 50mg/kg in saline; i.p.) administration. The age-matched control animals were injected with saline only. After 2 weeks, each animal was used for labeling of Abd motoneurons. All animals were anesthetized with Halothane (0.5-1%). Abd muscle nerve was sectioned and dipped for 2 hrs into a small polyethylene tube containing 10% dextran-fluorescein dissolved in saline. After 3 weeks survival period, the animals were re-anesthetized and perfused. The spinal cord was sectioned serially, observed with fluorescence microscopy, and the number and size of cell bodies were examined.
The mean number of labeled Abd motoneuorns was significantly less in the 2-week diabetic rats as compared with control animals. The mean soma diameter of Abd motoneurons was also significantly smaller in the 2-week diabetic animals. We conclude that chronic hyperglycemia induce the reduction of number and size of Abd motoneurons in only 2 weeks after STZ injection.

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