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突起伸展・回路形成 1
Axonal/Dendritic Growth and Circuit Formation 1

開催日 2014/9/13
時間 15:00 - 16:00
会場 Room J(313+314)
Chairperson(s) 古川 貴久 / Takahisa Furukawa (大阪大学蛋白質研究所 分子発生学研究室 / Laboratory for Molecular and Developmental Biology, Institute for Protein Research, Osaka University, Japan)
大森 義裕 / Yoshihiro Omori (大阪大学蛋白質研究所 / Institute for Protein Research, Osaka University, Japan)

Rabies Virus Hijacks and Accelerates the p75NTR Retrograde Axonal Transport Machinery

  • O3-J-3-4
  • Shani Gluska:1 Eitan Erez Zahavi:1 Michael Chein:1 Tal Gradus:1 Anja Bauer:2 Stefan Erez Finke:2 Eran Perlson:1 
  • 1: Tel Aviv University, Israel 2:Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Molecular Virology and Cell Biology, Germany 

Rabies virus (RABV) is a neurotropic virus that depends on long distance axonal transport in order to reach the central nervous system (CNS). The strategy RABV uses to hijack the cellular transport machinery is unknown. Here we use live cell imaging to track RABV entry at nerve terminals and study its retrograde transport along the axon, in a microfluidic chamber system. First, we demonstrate that RABV interacts with the p75 neurotrophin receptor (p75NTR) at peripheral neuron tips to enter the axon. Then, characterizing RABV retrograde transport along the axon, we showed the virus moving in acidic compartments, mostly with p75NTR. Interestingly, RABV is transported faster than NGF, the p75NTR endogenous ligand. Finally, we determine that p75NTR-dependent transport of RABV is faster and more directed than p75NTR-independent RABV transport. This fast route to the CNS is characterized by both an increase in instantaneous velocities and fewer stops en route. Hence, RABV-p75NTR dependent transport is a fast mechanism that can facilitate arrival of RABV to the CNS.

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